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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important purpose in advertising and marketing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two further splice variants had been reported but in-depth nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind to the epitope over the goal protein. By comparing the staining with the blocked antibody versus the antibody by itself, you can see which staining is precise.
Focus on Info Cyclin E belongs for the very conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show distinct expression and degradation designs which lead for the temporal coordination of each mitotic party. Cyclin E kinds get more info a complex with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates at the G1-S period boundary and is particularly degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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